The main purpose of this study is to analyze the relation between working capital and profitability in the agribusiness sector companies in comparison with other Brazilian listed companies. Differently from the previous studies, the central hypothesis sought to investigate whether the negative relation between the working capital and the profitability of the companies applies to companies in this sector and included another variable in the traditional model, the operating working capital requirement. Additionally, we tried to analyze the working capital quarterly seasonality effect on the profitability and the impact of the working capital management on the profitability of companies of the agribusiness sector in periods of economic recession. The regressions have been estimated on a quarterly basis for the period from 2007 to 2015. The results found present evidences that suggest the following conclusions: 1) given the specificity of the agribusiness sector, the cash conversion cycle does not explain the relation between profitability and management of working capital of these companies; 2) The operating working capital requirement presents statistical significance and an additional investment of this variable can add value to companies of that sector; 3) the seasonality influences the agribusiness companies working capital management and profitability and may add greater value in the two last quarters of the year; and 4) the efficient working capital management in recession times increases the companies profitability.